Targeting DNA double-strand breaks with TAL effector nucleases.
نویسندگان
چکیده
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
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ورودعنوان ژورنال:
- Genetics
دوره 186 2 شماره
صفحات -
تاریخ انتشار 2010